The laboratory specializes in identification and quantification of small molecules and metabolites utilizing targeted MRM assays in complex biological samples. Conducts analysis of key cellular metabolite pathway using targeted MRM panel assays. And carries out untargeted metabolomics profiling in variety of biological matrixes.
Targeted Metabolite Analysis:
- Relative and absolute quantification of metabolites using MRM based targeted assay incorporating isotopic standards for increased identification and quantification accuracy.
- Develops and establishes custom targeted metabolite assays for small molecules and metabolites.
- Analysis of entire metabolic pathways using targeted MRM panel assays covering key pathways involved in the metabolism of cells and cancer.
- Over 265 available, absolute quantification targeted MRM assays spanning many of the key cellular metabolite pathways such as the glycolysis, pentose phosphate, TCA, oxidative phosphorylation, amino acids and numerus derivatives, nucleotides (purine and pyrimidine) and short- medium and long chain fatty acids for example.
- Identification and quantitation of steroid hormones (estrogen, progesterone, and androgen) in different biological matrixes.
- Identification and quantification of native and modified nucleotides (DNA and RNA).
- Pharmacokinetics (PK) analysis, develop quantitative MRM assay for known and novel drugs and small molecules. Analyzing drug metabolites in relevant biofluids and tissues.
- Purity determination and structural analysis of small molecules.
- Isotope tracing to monitor metabolite pathway activities.
Global Untargeted Metabolomics Analysis:
- Each sample is analyzed under two different liquid chromatographic (LC) conditions, Reverser Phase (RP) and Hydrophilic interaction chromatography (HILIC) which is ran under two different pH conditions (pH3 and pH9). For both LC methods the mass spectrometry data is acquired under both negative and positive ionization mode. Spiked in isotopic standards, water blanks, pool samples and reference samples are used for data filtration, normalization, and QC assessment.
- Raw mass spectrometry data search is performed in Compound Discoverer, followed by post search analysis in excel and R.