The laboratory specializes in development of assays for identification and quantification of small molecules and metabolites in complex biological samples. The lab also conducts analysis of key cellular metabolite pathways using targeted panel assays and carries out untargeted metabolomics profiling in variety of biological matrixes.
Targeted Metabolite Analysis:
- Relative and absolute quantification of metabolites using MRM based targeted assay incorporating isotopic standards for increased identification and quantification accuracy.
- Develops and establishes custom targeted metabolite assays for small molecules and metabolites.
- Analysis of entire metabolic pathways using targeted MRM panel assays covering key pathways involved in the metabolism of cells and cancer.
- Over 265 available, absolute quantification targeted MRM assays spanning many of the key cellular metabolite pathways such as the glycolysis, pentose phosphate, TCA, oxidative phosphorylation, amino acids and numerus derivatives, nucleotides (purine and pyrimidine) and short- medium and long chain fatty acids for example.
- Identification and quantitation of steroid hormones (estrogen, progesterone, and androgen) in different biological matrixes.
- Identification and quantification of native and modified nucleotides (DNA and RNA).
- Pharmacokinetics (PK) analysis, develop quantitative MRM assay for known and novel drugs and small molecules. Analyzing drug metabolites in relevant biofluids and tissues.
- Purity determination and structural analysis of small molecules.
- Isotope tracing to monitor metabolite pathway activities.
Global Untargeted Metabolomics Analysis:
- Support for Untargeted Metabolomics can be requested through the NIH Metabolomics Consortium (NIH-MC).