The Protein Expression Laboratory generated a variety of novel protein production technologies to improve protein yields and quality, and reduce the time needed to generate protein reagents. We specialize in challenging protein targets, including multiprotein complexes for structural biology and assay development, along with microscale technologies to minimize the cost and effort in protein production screening while allowing us to test larger numbers of proteins to identify optimal choices before large-scale production.
Structural Biology Applications:
We produce high-quality individual recombinant proteins and protein complexes and optimize protein constructs using microscale expression and purification to quickly identify the optimal protein domains for production. Proteins can readily be labelled with isotopes. Applications include:
- X-ray crystallography
- Nuclear magnetic resonance
- Cryo-electron microscopy
Drug Discovery Assay Development and Deployment:
We generate proteins with a variety of domain designs and tagging strategies to support biochemical and biophysical drug discovery assays. Our collection of more than 100 different fusion protein tags ranges from various fluorescent proteins to antibody-based detection tags.
- Surface plasmon resonance
- Homogenous time-resolved fluorescence
- Fluorescence polarization
- Isothermal calorimetry
New Technologies for Enhanced Protein Production:
We develop new reagents to improve production of proteins from insect cells and use specialized cloning processes to permit multi-gene assembly assist in the production of large multiprotein complexes for structural biology and drug discovery. We use magnetic bead technologies to accelerate protein purification timelines and reduce cost, as well as new expression systems to provide additional options for challenging protein targets.
Applications Include:
- Novel high-producing cell lines
- Modified DNA constructs for faster and more efficient baculovirus production
- Chaperones to assist with better recombinant protein folding