Announcement:
FNL Couriers no longer accepted pickup/delivery requests via the phone or email. All FNL Courier pickups from Bethesda to Frederick, must be submitted through the Ship Wizard system. All shipments that are transported on the highway (FNL Courier pickups) must be classified as a Hazardous or Non Hazardous shipment and the only way to have that done is to submit a Request for Shipment in the Ship Wizard system.
Ship Wizard Link: https://ncifrederick.cancer.gov/Cad/ShippingWizard
This is a free service, but you need to enter your PID number when filling out the form. If you do not know your PID, you can check with your AO or this information will also be in your NAS request.
To request services from the CCR Sequencing Facility
Submit a Sequencing Facility Request
Prior to filling out a NAS request, you are advised to consult with Bao Tran to discuss your project design and bioinformatics approach to data analysis:
Bao Tran
Director, Sequencing Facility
bao.tran@nih.gov
301-360-3460
Please visit the Protocols and Resources page for more details about the sequencing chemistry and technology utilized by each platform. We encourage you to contact us so we can provide you with the most current information and help you plan your project to meet your sequencing needs.
Short reads with Illumina Sequencing:
Illumina sequencing enables a wide variety of applications, allowing researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism.
- ChIP-Seq
- Cut and Run
- ATAC-Seq*
- RNA-Seq (mRNA, Total RNA and microRNA)
- Whole Genome Sequencing
- Whole Exome Sequencing – For further information please contact NCI-FredLMTSFExome@mail.nih.gov.
- Methylated DNA sequencing (bisulfite)
- Amplicon Sequencing
* ATAC seq is only provided as a pilot project for a maximum of 12 samples. After the pilot, or for more than 12 samples, we can transfer the protocol to you.
Long Read Sequencing Techonologies:
- Whole Genome Sequencing: de novo assembly, haplotype resolution, structural variant detection, DNA epigenetic modification detection.
- RNA Sequencing: Full-length transcript sequencing for whole-transcriptome or gene-specific targets. Full-length RNA sequencing can be performed on bulk or single cell samples. Direct RNA sequencing with Oxford Nanopore Sequencing.
- Targeted Sequencing: Long amplicon sequencing, full-length viral sequencing, full-length vector sequencing, target enrichment, adaptive sampling and multiplexing strategies.
- HLA Typing: Amplification of full gene for HLA class I and/or class II.
- 16S sequencing: Amplification of full length 16S for bacterial communities.
Optical Mapping using Bionano Technology:
Imaging and analysis of extremely long, high-molecular-weight DNA facilitates identification of structural variants and creation of de novo genome assemblies
R&D Resources:
- R&D group works closely with investigators to provide customized support for a variety of applications, utilizing the most recent state-of-the-art NGS sequencing technologies.
- Testing and validation of new sequencing applications/products before offering them as production services.
- Development of new sequencing applications/protocols to assist the NCI community.
- Training the production team members and PI labs on the newest developed NGS sequencing technologies and new instruments.
Bioinformatics Support:
We specialize in next-generation sequencing data analysis and quality control, sequencing technology consultation, assessment and validation of new protocols and technologies, and data analysis and management. We support analysis for Whole Genome/Exome sequencing, bulk and single cell transcriptomic sequencing, bulk and single cell epigenetics sequencing from short-read and long-read sequencing platforms. We work collaboratively with the CCR Collaborative Bioinformatics Resource (CCBR) and bioinformaticians at CCR investigator labs to provide a mechanism for researchers to obtain many different types of bioinformatics assistance to further their research goals. Please check the details of Bioinformatics Support at the Bioinformatics page.
Bioinformatics inquiries can be mailed to Yongmei Zhao (yongmei.zhao@nih.gov) or CCRSF_IFX@nih.gov.