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To Request Service from the Sequencing Facility

Prior to filling out a NAS request, you are advised to consult with Mr. Bao Tran  and/or Dr. Maggie Cam to discuss your project design and bioinformatics approach to data analysis:

1

Bao Tran,

Sequencing Facility Head

tranb2@mail.nih.gov

301-360-3460

2

Maggie Cam Ph.D.,

Staff Scientist

maggie.cam@nih.gov

301-443-2965

3

Request Service

Submit a NAS Request

Please visit the Protocols and Resources page for more details about the sequencing chemistry and technology utilized by each platform. We encourage you to contact us so we can provide you with the most current information and help you plan your project to meet your sequencing needs.

Long-reads/PacBio Sequencing

If you want more information on long reads technologies, please contact us at LongReadInfo@mail.nih.gov

Whole Genome Sequencing

de novo assembly, haplotype resolution, structural detect, DNA epigenetic modification detection.

RNA Sequencing

full-length transcript sequencing for whole-transcriptome or gene-specific targets.

Targeted Sequencing

long amplicon sequencing; full-length viral sequencing, full-length vector sequencing, targeted enrichment, and multiplexing strategies.

HLA Typing

amplification of full gene for HLA class I, other HLA genes target available.

Single-Cell Sequencing

Single Cell Transcriptomics

3’ and 5’ gene expression profiling to uncover cell-to-cell gene expression variability.

Single Cell Immune Profiling

simultaneous analysis of the cellular context of the adaptive immune response and immune repertoires of thousands of T and B cells.

Single Cell ATAC-seq

chromatin profiling of thousands of single cells to provide insights into the regulatory landscape of the genome.

Single Cell CNV Analysis

single cell CNV calls at 2 Mb resolution for revealing genome heterogeneity.

Other Sequencing Services

Whole Genome Sequencing using 10x Genomics Linked-Reads

Long-range information from short-read sequencing, to resolve genic phasing, reveal structural variation and detect variants in complex genomic regions.

Optical mapping using Bionano Technology

Imaging and analysis of extremely long, high-molecular-weight DNA felicitates identification of structural variants and creation of de novo genome assemblies.

Exome Sequencing using 10x Genomics Linked-Reads

Large scale structural variants detection across the entire exome.

Bioinformatics Support

CCR-SF bioinformatics group provides coordinated joint consultation services for sequencing technology selection, project design, and data analysis for next generation sequencing projects. We support analysis for Whole Genome/Exome sequencing, ATAC-seq, ChIP-seq, RNA-seq, miRNA-seq and analysis for new data types from single cell sequencing, long-read sequencing, and optical mapping. We work collaboratively with the CCR Collaborative Bioinformatics Resource (CCBR) and provide a mechanism for CCR researchers to obtain many different types of bioinformatics assistance to further their research goals.