2024
Centrosome amplification and aneuploidy driven by the HIV-1-induced Vpr•VprBP•Plk4 complex in CD4+ T cells
Park JE, Kim TS, Zeng Y, Mikolaj M, Il Ahn J, Alam MS, Monnie CM, Shi V, Zhou M, Chun TW, Maldarelli F, Narayan K, Ahn J, Ashwell JD, Strebel K, Lee KS. (2024). Nature Communications. 15(1):2017. doi: 10.1038/s41467-024-46306-8.
A spatial map of hepatic mitochondria uncovers functional heterogeneity shaped by nutrient-sensing signaling
Kang SWS, Cunningham RP, Miller CB, Brown LA, Cultraro CM, Harned A, Narayan K, Hernandez J, Jenkins LM, Lobanov A, Cam M, Porat-Shliom N. (2024 Feb 28) Nature Communications. 15(1):1799. doi: 10.1038/s41467-024-45751-9.
Reconstruction via Manual and Machine-Learning Aided Segmentation of Volume EM Datasets.
Vijayakumaran A, Abuammar A, Medagedara O, Narayan K, Mennella V. (2024) Methods Mol Biol.;2725:131-146. doi: 10.1007/978-1-0716-3507-0_8.
2023
CDK2 inhibition disorders centrosome stoichiometry and alters cellular outcomes in aneuploid cancer cells.
Chen Z, Liu X, Kawakami M, Liu X, Baker A, Bhatawadekar A, Tyutyunyk-Massey L, Narayan K, Dmitrovsky E.
(2023 Dec 31) Cancer Biol Ther.; 24(1):2279241. doi: 10.1080/15384047.2023.2279241.
Volume EM: a quiet revolution takes shape
Collinson LM, Bosch C, Bullen A, Burden JJ, Carzaniga R, Cheng C, Darrow MC, Fletcher G, Johnson E, Narayan K, Peddie CJ, Winn M, Wood C, Patwardhan A, Kleywegt GJ, Verkade P.
Nat Methods. 2023 Jun;20(6):777-782. doi: 10.1038/s41592-023-01861-8.
Non-specific and specific DNA binding modes of bacterial histone, HU, separately regulate distinct physiological processes through different mechanisms
Verma, S. C., Harned, A., Narayan, K., & Adhya, S.
(2023). Molecular Microbiology. https://doi.org/10.1111/MMI.15033
Instance segmentation of mitochondria in electron microscopy images with a generalist deep learning model trained on a diverse dataset
Conrad, R., & Narayan, K. (2023). Cell Systems, 14(1), 58-71. e5. //doi.org/10.1016/J.CELS.2022.12.006
2022
Delivery of ceramide phosphoethanolamine lipids to the cleavage furrow through the endocytic pathway is essential for male meiotic cytokinesis
Kunduri, G., Le, S.-H., Baena, V., Vijaykrishna, N., Harned, A., Nagashima, K., Blankenberg, D., Yoshihiro, I., Narayan, K., Bamba, T., Acharya, U., & Acharya, J. K. (2022). PLOS Biology, 20(9), e3001599. doi.org/10.1371/journal.pbio.3001599
A versatile enhanced freeze-substitution protocol for volume electron microscopy
Bélanger, S., Berensmann, H., Baena, V., Duncan, K., Meyers, B. C., Narayan, K., & Czymmek, K. J. (2022).
Frontiers in Cell and Developmental Biology, 10. doi.org/10.3389/fcell.2022.933376
Volume electron microscopy
Peddie, C. J., Genoud, C., Kreshuk, A., Meechan, K., Micheva, K. D., Narayan, K., Pape, C., Parton, R. G., Schieber, N. L., Schwab, Y., Titze, B., Verkade, P., Weigel, A., & Collinson, L. M. (2022). Nature Reviews Methods Primers, 2(1), 51. https://doi.org/10.1038/s43586-022-00131-9
Intracellular connections between basal bodies promote the coordinated behavior of motile cilia
Soh AWJ, Woodhams LG, Junker AD, Enloe CM, Noren BE, Harned A, Westlake CJ, Narayan K, Oakey JS, Bayly PV, Pearson CG.
Mol Biol Cell. 2022 Sep 15:mbcE22050150. doi: 10.1091/mbc.E22-05-0150.
Embedding of HIV Egress within Cortical F-Actin
Anupriya Aggarwal , Alberto Ospina Stella , Catherine C Henry , Kedar Narayan , Stuart G Turville. Pathogens. 2022 Jan 3;11(1):56. doi: 10.3390/pathogens11010056
2021
REMBI: Recommended Metadata for Biological Images-enabling reuse of microscopy data in biology
Sarkans U, Chiu W, Collinson L, Darrow MC, Ellenberg J, Grunwald D, Hériché JK, Iudin A, Martins GG, Meehan T, Narayan K, Patwardhan A, Russell MRG, Saibil HR, Strambio-De-Castillia C, Swedlow JR, Tischer C, Uhlmann V, Verkade P, Barlow M, Bayraktar O, Birney E, Catavitello C, Cawthorne C, Wagner-Conrad S, Duke E, Paul-Gilloteaux P, Gustin E, Harkiolaki M, Kankaanpää P, Lemberger T, McEntyre J, Moore J, Nicholls AW, Onami S, Parkinson H, Parsons M, Romanchikova M, Sofroniew N, Swoger J, Utz N, Voortman LM, Wong F, Zhang P, Kleywegt GJ, Brazma A.
Nat Methods. 2021 May 21. doi: 10.1038/s41592-021-01166-8.
FIB-SEM as a Volume Electron Microscopy Approach to Study Cellular Architectures in SARS-CoV-2 and Other Viral Infections: A Practical Primer for a Virologist
Baena V, Conrad R, Friday P, Fitzgerald E, Kim T, Bernbaum J, Berensmann H, Harned A, Nagashima K, Narayan K.
Viruses. 2021 Apr 2;13(4):611. doi: 10.3390/v13040611.
CEM500K, a large-scale heterogeneous unlabeled cellular electron microscopy image dataset for deep learning
Conrad R, Narayan K.
Elife. 2021 Apr 8;10:e65894.
A Workflow for High-pressure Freezing and Freeze Substitution of the Caenorhabditis elegans Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy
Rahman MM, Chang IY, Cohen-Fix O, Narayan K.
Bio Protoc. 2021 Apr 5;11(7):BioProtoc.3981. eCollection 2021 Apr 5.
Cryo-fluorescence microscopy of high-pressure frozen C. elegans enables correlative FIB-SEM imaging of targeted embryonic stages in the intact worm
Chang IY, Rahman M, Harned A, Cohen-Fix O, Narayan K.
Methods Cell Biol. 2021;162:223-252. Epub 2020 Nov 4.
2020
C. elegans pronuclei fuse after fertilization through a novel membrane structure
Rahman M, Chang IY, Harned A, Maheshwari R, Amoateng K, Narayan K, Cohen-Fix O.
Cell Biol. 2020 Feb 3;219(2).
Efficient Skeleton Editing in a VR Environment Facilitates Accurate Modeling of Highly Branched Mitochondria
Conrad, R., Ruth, T., Löffler, F., Hadlak, S., Konrad, S., Götze, C., . . . Narayan, K. Microscopy and Microanalysis, 1-4. (2020)
Enforcing Prediction Consistency Across Orthogonal Planes Significantly Improves Segmentation of FIB-SEM Image Volumes by 2D Neural Networks
Conrad, R., Lee, H., & Narayan, K. Microscopy and Microanalysis, 1-4. (2020)
