Supported by CCR Office of Science and Technology Resources (OSTR)

Next-Gen Sequencing Services

To request services from the FSGC Next-Gen Sequencing Group
Submit a Sequencing Request
Prior to filling out a NAS request, you are advised to consult with Bao Tran to discuss your project design and bioinformatics approach to data analysis:

Bao Tran
Director, Next-Gen Sequencing Group
bao.tran@nih.gov
301-360-3460

Please visit the Protocols and Resources page for more details about the sequencing chemistry and technology utilized by each platform. We encourage you to contact us so we can provide you with the most current information and help you plan your project to meet your sequencing needs.

Long Read Sequencing Techonologies

The following methodologies encompass a diverse range of advanced sequencing techniques and applications used for genomic research, including whole genome sequencing, RNA sequencing, targeted sequencing, and 16S sequencing.

  • Whole Genome Sequencing: de novo assembly, haplotype resolution, structural variant detection, DNA epigenetic modification detection.
  • RNA Sequencing: Full-length transcript sequencing for whole-transcriptome or gene-specific targets. Full-length RNA sequencing can be performed on bulk or single cell samples. Direct RNA sequencing with Oxford Nanopore Sequencing.
  • Targeted Sequencing: Long amplicon sequencing, full-length viral sequencing, full-length vector sequencing, target enrichment, adaptive sampling and multiplexing strategies.
  • 16S sequencing: Amplification of full length 16S for bacterial communities.

Short Reads with Illumina Sequencing

Illumina sequencing enables a wide variety of applications, allowing researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism.

  • Cut and Run
  • RNA-Seq (mRNA, Total RNA and microRNA)
  • Whole Genome Sequencing
  • Whole Exome Sequencing – For further information please contact NCI-FredLMTSFExome@mail.nih.gov.
  • Methylated DNA sequencing (bisulfite)
  • Amplicon Sequencing
  • We do accept ready-to-sequence Illumina-compatible libraries for sequencing. To ensure the quality of the data, please check the core’s recommended guidelines.

Single Cell Sequencing*

We offer end-to-end single cell sequencing solutions utilizing the latest technologies including 10X genomics and PIP-Seq. We also offer single-cell full-length Iso-seq using PacBio Kinnex or Oxford Nanopore platforms.

  • 10X Genomics
    • GEM-X 3’ and 5’ RNA_Seq
    • Chromium HT 3’ and 5’ RNA-Seq
    • VDJ Enrichment
    • Single Cell ATAC-seq
    • Fixed RNA-Seq
  • BD Rhapsody
    • Immune Profiling
*Please note that FSGC-NGS can only provide the full single-cell service (cell capture + sequencing) for labs located on the Frederick Campus.

Bioinformatics Support

FSGC Next-Gen Sequencing Bioinformatics group provides coordinated joint consultation services for sequencing technology selection, project design, and data analysis for next generation sequencing projects. We support analysis for Whole Genome/Exome sequencing, RNA-seq, miRNA-seq and analysis for new data types from single cell sequencing, long-read sequencing and optical mapping. We work collaboratively with the CCR Collaborative Bioinformatics Resource (CCBR) and provide a mechanism for CCR researchers to obtain many different types of bioinformatics assistance to further their research goals.

Sequencing Cost Calculator