Supported by CCR Office of Science and Technology Resources (OSTR)

Next-Gen Sequencing Pricing

Get an instant pricing estimate* on your next sequencing project.

Illumina Library ConstructionNCI/NIAID PriceNON NCI Price
Project TypeCost/SampleCost/Sample
gDNA-Seq$68 + (Sequencing Cost)$136 + ( Sequencing Cost)
Nextera XT$65 + (Sequencing Cost)$130 + ( Sequencing Cost)
Whole Genome Methyl-Seq$85 + (Sequencing Cost)$170 + ( Sequencing Cost)
5hmC Detection$102 + ( Sequencing Cost)$205 + ( Sequencing Cost)
Total RNA-Seq$84 + ( Sequencing Cost)$168 + ( Sequencing Cost)
mRNA-Seq$60 + ( Sequencing Cost)$120 + ( Sequencing Cost)
miRNA-Seq$108 + ( Sequencing Cost)$218 + ( Sequencing Cost)
Single Cell 10X Library ConstructionNCI/NIAID PriceNON NCI Price
Project TypeCost/SampleCost/Sample
10X Chromium GEM-X Single Cell RNA_Seq (GEX, for 3′ V4 or 5′ V3)$1614+ (Sequencing Cost)$3228 + ( Sequencing Cost)
10X Chromium Next GEM Single Cell RNA_Seq (GEX, for 3′ V3.1 or 5′ V2)$1785 + (Sequencing Cost)$3570 + ( Sequencing Cost)
10X Chromium Single Cell VDJ Enrichment (TCR or BCR, for 5’ only)$125 + (10X Capture) + (Sequencing Cost)$250 + (10X Capture) + (Sequencing Cost)
10X Chromium Single Cell Feature Barcode (for 3’ or 5’)$146 + (10X Capture) + (Sequencing Cost)$292 + (10X Capture) + (Sequencing Cost)
10X Chromium Single Cell  ATAC-seq$1700 + (Sequencing Cost)$3400 + (Sequencing Cost)
10X Chromium HT Single Cell RNA_Seq (GEX, for 3′ or 5′)$1921 + (Sequencing Cost)$3842 + (Sequencing Cost)
10X Chromium Fixed RNA_Seq for Multiplexed Sample (1rxn x 4 BC)$3894 + (Sequencing Cost)$7788 + (Sequencing Cost)
10X Chromium Fixed RNA_Seq for Singleplexed sample$1873 + (Sequencing Cost)$3746 + (Sequencing Cost)
Single Cell Fluent PIPSeq 3′ Library ConstructionNCI/NIAID PriceNON NCI Price
Project TypeCost/SampleCost/Sample
Fluent BioSciences PIPseq T2$324 +  (Sequencing Cost))$648 + (Sequencing Cost)
Fluent BioSciences PIPseq T10$692 + (Sequencing Cost)$1384 + (Sequencing Cost)
Fluent BioSciences PIPseq T20$954 + (Sequencing Cost)$1908 + (Sequencing Cost)
Fluent BioSciences PIPseq T100$3369 + (Sequencing Cost)$6738 + (Sequencing Cost)

Illumina Sequencing

NovaSeq X SeriesNCI/NIAID PriceNON NCI Price
Run TypeCost/FlowcellCost/Flowcell
X Plus 1.5B 100 Cycle$1,929$3,857
X Plus 1.5B 200 Cycle$2,436$4,872
X Plus 1.5B 300 Cycle$2,639$5,278
X Plus 10B 100 cycle$7,196$14,392
X Plus 10B 200 cycle$9,122$18,243
 X Plus 10B 300 cycle$9,730$19,459
 X Plus 25B 100 cycle$11,876$23,752
 X Plus 25B 300 cycle$16,240$32,480
NextSeq 2000NCI/NIAID PriceNON NCI Price
Run TypeCost/FlowcellCost/Flowcell
P1 100 cycle$731$1,462
P2 100 Cycle$1,108$2,217
P2 200 Cycle$2,084$4,168
P2 300 Cycle$2,763$5,526
P2 600 Cycle$3,007$6,015
P3 100 cycle$2,367$4,734
P3 200 cycle$3,347$6,695
P3 300 cycle$4,370$8,739
P4 100 cycle$2,741$5,481
P4 200 cycle$3,875$7,751
P4 300 cycle$4,933$9,866
iSeqNCI/NIAID PriceNON NCI Price
Run TypeCost/FlowcellCost/Flowcell
iSeq 100 i1 Reagent v2$652.69$1,305.38
Pacbio Library ConstructionNCI/NIAID PriceNON NCI Price
Project TypeCost/SampleCost/Sample
Amplicon$48 + (Sequencing Cost)$96 + (Sequencing Cost)
Iso-seq$86 + (Sequencing Cost)$172 + (Sequencing Cost)
WGS$147 + (Sequencing Cost)$294 + (Sequencing Cost)
HLA- multiplex class I and II$146 + (Sequencing Cost)$292 + (Sequencing Cost)
Full-length 16S$7 + (Sequencing Cost)$14 + (Sequencing Cost)
Single Cell MAS Iso-seq *$485 + (Sequencing Cost)$970 + (Sequencing Cost)
Ultra-low Input (PCR-based)$263 + (Sequencing Cost)$526 + (Sequencing Cost)
Targeted Iso-seq **$225 + (Sequencing Cost)$450 + (Sequencing Cost)
Xdrop Sort$580 + (Sequencing Cost)$1160 + (Sequencing Cost)
* Single cell capture is not included
** Probes cost is not included
Pacbio SequencingNCI/NIAID PriceNON NCI Price
Project TypeCost/SMRT CellCost/Flowcell
Revio SMRT Cell$1,010$2,020
Oxford Nanopore Library ConstructionNCI/NIAID PriceNON NCI Price
Project TypeCost/SampleCost/Sample
Nanopore WGS$169 + (Sequencing Cost)$338 + (Sequencing Cost)
Nanopore Targeted WGS (adaptive sampling)$172 + (Sequencing Cost)$343 + (Sequencing Cost)
Nanopore Direct RNA-Seq$143 + (Sequencing Cost)$286 + (Sequencing Cost)
Nanopore Ultra-Long$185 + (Sequencing Cost)$370 + (Sequencing Cost)
Nanopore RNA-seq (cDNA-PCR)$150 + (Sequencing Cost)$300 + (Sequencing Cost)
Nanopore RNA-seq Multiplexing (cDNA-PCR)$175 + (Sequencing Cost)$350 + (Sequencing Cost)
Nanopore Single cell transcriptomics $165 + (Sequencing Cost)$330 + (Sequencing Cost)
Oxford Nanopore SequencingNCI/NIAID PriceNON NCI Price
Project TypeCost/FlowcellCost/Flowcell
Nanopore flow cell$913$1,826
Washing kit$17$34
Reloading reagents$9$17

Sequencing Cost Calculator