Supported by CCR Office of Science and Technology Resources (OSTR)

Next-Gen Sequencing Pricing

Get an instant pricing estimate* on your next sequencing project.

Illumina Library ConstructionNCI/NIAID PriceNON NCI Price
Project TypeCost/SampleCost/Sample
gDNA-Seq*$73 + (Seq Cost)$146 + ( Seq Cost)
Nextera XT$72 + (Seq Cost)$144 + ( Seq Cost)
Whole Genome Methyl-Seq*$85 + (Seq Cost)$170 + ( Seq Cost)
Methyl-Seq with 5-hmC Detection $103 + ( Seq Cost)$206 + ( Seq Cost)
Total RNA-Seq*$91 + ( Seq Cost)$182 + ( Seq Cost)
mRNA-Seq*$70 + ( Seq Cost)$140 + ( Seq Cost)
miRNA-Seq*$135 + ( Seq Cost)$270 + ( Seq Cost)

*These services are only avalible for “non-routine” project
Single Cell 10X Chromium Library ConstructionCCR/NIAID PriceNON NCI Price
Project TypeCost/SampleCost/Sample
GEM-X assay
GEM-X Gene Expression$2025 + (Sequencing Cost)$4050 + ( Sequencing Cost)
GEM-X  VDJ Enrichment$111 + (10X Capture) +(Sequencing Cost)$222 + (10X Capture) +(Sequencing Cost)
GEM-X Feature Barcode $130 + (10X Capture) + (Sequencing Cost)$260 + (10X Capture) + (Sequencing Cost)
GEM-X Universal On-Chip-Multiplex (4-plex)$887 + (Sequencing Cost)$1774 + (Sequencing Cost)
GEM-X  Flex Gene Expression 1×4 (1-plex)$2078 + (Sequencing Cost)$4156 + (Sequencing Cost)
GEM-X  Flex Gene Expression 4×4 (4-plex)$1103 + (Sequencing Cost)$2206 + (Sequencing Cost)
Next GEM assay
Next GEM Gene Expression$2206 + (Sequencing Cost)$4412 + (Sequencing Cost)
Next GEM  VDJ Enrichment$111 + (10X Capture) + (Sequencing Cost)$222 + (10X Capture) + (Sequencing Cost)
Next GEM  CRISPR$130 + (10X Capture) + (Sequencing Cost)$260 + (10X Capture) + (Sequencing Cost)
Next GEM Feature Barcode $127 + (10X Capture) + (Sequencing Cost)$254 + (10X Capture) + (Sequencing Cost)
Next GEM Multiome ATAC + Gene Expression$3480 + (Sequencing Cost)$6960 + (Sequencing Cost)
Next GEM ATAC$2040 + (Sequencing Cost)$4080+ (Sequencing Cost)
Single Cell Illumina PIPSeq Library ConstructionNCI/NIAID PriceNON NCI Price
Project TypeCost/SampleCost/Sample
Illumina PIPseq T2$324 +  (Sequencing Cost))$648 + (Sequencing Cost)
Illumina PIPseq T10$692 + (Sequencing Cost)$1384 + (Sequencing Cost)
Illumina PIPseq T20$954 + (Sequencing Cost)$1908 + (Sequencing Cost)
Illumina PIPseq T100$3369 + (Sequencing Cost)$6738 + (Sequencing Cost)
NovaSeq X SeriesNCI/NIAID PriceNON NCI Price
Run TypeCost/FlowcellCost/Flowcell
X Plus 1.5B 100 Cycle$1,983$3,993
X Plus 1.5B 200 Cycle$2,505$5,044
X Plus 1.5B 300 Cycle$2,714$5,464
X Plus 10B 100 cycle$7,412$14,922
X Plus 10B 200 cycle$9,395$18,915
 X Plus 10B 300 cycle$10,021$20,176
 X Plus 25B 100 cycle$11,876$23,927
 X Plus 25B 200 cycle$14,718$29,653
X Plus 25B 300 cycle$16,702$33,627
NextSeq XLEAPNCI/NIAID PriceNON NCI Price
Run TypeCost/FlowcellCost/Flowcell
P1 100 cycle$730$1,450
P1 300 cycle$1,014$2,014
P1 600 cycle$1,541$3,061
P2 100 cycle$1,107$2,199
P2 200 cycle$2,081$4,134
P2 300 cycle$2,759$5,481
P2 600 cycle$3,003$5,966
P3 100 cycle$2,363$4,695
P3 200 cycle$3,343$6,642
P3 300 cycle$4,363$8,668
P4 50 cycle$1,824$3,624
P4 100 cycle$2,736$5,436
P4 200 cycle$3,780$7,688
P4 300 cycle$4,926$9,786
iSeqNCI/NIAID PriceNON NCI Price
Run TypeCost/FlowcellCost/Flowcell
iSeq 100 i1 Reagent v2$682$1,355
Pacbio Library ConstructionNCI/NIAID PriceNON NCI Price
Project TypeCost/SampleCost/Sample
Amplicon$76 + (Sequencing Cost)$152 + (Sequencing Cost)
Bulk Iso-seq$200 + (Sequencing Cost)$400 + (Sequencing Cost)
Bulk Kinnex Iso-seq$606+ (Sequencing Cost)$1213 + (Sequencing Cost)
WGS$147 + (Sequencing Cost)$294 + (Sequencing Cost)
Full-length 16S$7 + (Sequencing Cost)$14 + (Sequencing Cost)
Single Cell Kinney Iso-seq *$485 + (Sequencing Cost)$970 + (Sequencing Cost)
Targeted Iso-seq **$225 + (Sequencing Cost)$450 + (Sequencing Cost)
* Single cell capture is not included
** Probes cost is not included
Pacbio SequencingNCI/NIAID PriceNON NCI Price
Project TypeCost/SMRT CellCost/Flowcell
Revio SPRQ SMRT Cell$1,010$2,020
Oxford Nanopore Library ConstructionNCI/NIAID PriceNON NCI Price
Project TypeCost/SampleCost/Sample
Nanopore WGS$169 + (Sequencing Cost)$338 + (Sequencing Cost)
Nanopore Targeted WGS (adaptive sampling)$172 + (Sequencing Cost)$344 + (Sequencing Cost)
Nanopore Direct RNA-Seq$143 + (Sequencing Cost)$286 + (Sequencing Cost)
Nanopore Ultra-Long$185 + (Sequencing Cost)$370 + (Sequencing Cost)
Nanopore RNA-seq (cDNA-PCR)$150 + (Sequencing Cost)$300 + (Sequencing Cost)
Nanopore RNA-seq Multiplexing (cDNA-PCR)$175 + (Sequencing Cost)$350 + (Sequencing Cost)
Nanopore Single cell transcriptomics $165 + (Sequencing Cost)$330 + (Sequencing Cost)
Oxford Nanopore SequencingNCI/NIAID PriceNON NCI Price
Project TypeCost/FlowcellCost/Flowcell
Nanopore flow cell$913$1,826
Washing kit$17$34
Reloading reagents$9$17

Sequencing Cost Calculator