Pricing Calculator
Get an instant pricing estimate* on your next sequencing project.
| Illumina Library Construction | NCI Price | Non NCI Price |
|---|---|---|
| Project Type | Cost/Sample | Cost/Sample |
| gDNA-Seq | $60 + (Sequencing Cost) | $120 + (Sequencing Cost) |
| Nextera XT | $57 + (Sequencing Cost) | $114 + (Sequencing Cost) |
| Whole Genome Methyl-Seq* | $116 + (Sequencing Cost) | $232 + (Sequencing Cost) |
| Total RNA-Seq | $91 + (Sequencing Cost) | $182 + (Sequencing Cost) |
| mRNA-Seq | $76 + (Sequencing Cost) | $152 + (Sequencing Cost) |
| miRNA-Seq | $137 + (Sequencing Cost) | $274 + (Sequencing Cost) |
| *Illumina 5-Base protocol, if you need other methylation protocol please discuss with the FSGC-NGS team | ||
| NovaSeq X Series | NCI Price | Non NCI Price |
|---|---|---|
| Run Type | Cost/Flowcell | Cost/Flowcell |
| X Plus 1.5B 100 cycle | $2,042 | $4,084 |
| X Plus 1.5B 200 cycle | $2,580 | $5,160 |
| X Plus 1.5B 300 cycle | $2,794 | $5,588 |
| X Plus 5B 100 cycle | $5,046 | $10,092 |
| X Plus 5B 200 cycle | $6,383 | $12,766 |
| X Plus 5B 300 cycle | $6,831 | $13,662 |
| X Plus 10B 100 cycle | $7,598 | $15,196 |
| X Plus 10B 200 cycle | $9,626 | $19,252 |
| X Plus 10B 300 cycle | $10,275 | $20,550 |
| X Plus 25B 100 cycle | $11,911 | $23,822 |
| X Plus 25B 200 cycle | $15,131 | $30,262 |
| X Plus 25B 300 cycle | $17,196 | $34,392 |
| NextSeq XLEAP | NCI Price | Non NCI Price |
|---|---|---|
| Run Type | Cost/Flowcell | Cost/Flowcell |
| P1 100 cycle | $755 | $1,510 |
| P1 300 cycle | $1,049 | $2,098 |
| P1 600 cycle | $3,107 | $6,214 |
| P2 100 cycle | $1,145 | $2,290 |
| P2 200 cycle | $2,153 | $4,306 |
| P2 300 cycle | $2,854 | $5,708 |
| P2 600 cycle | $3,107 | $6,214 |
| P3 100 cycle | $2,445 | $4,890 |
| P3 200 cycle | $3,386 | $6,772 |
| P3 300 cycle | $4,514 | $9,028 |
| P4 50 cycle | $1,887 | $3,774 |
| P4 100 cycle | $2,831 | $5,662 |
| P4 200 cycle | $4,004 | $8,008 |
| P4 300 cycle | $5,096 | $10,192 |
| iSeq | NCI Price | Non NCI Price |
|---|---|---|
| Run Type | Cost/Flowcell | Cost/Flowcell |
| iSeq 100 i1 Reagent v2 | $713 | $1,426 |
| Single Cell Services Single Cell 10X Library Construction | NCI Price | Non NCI Price |
|---|---|---|
| Project Type | Cost/Sample | Cost/Sample |
| GEM-X Universal Gene Expression | $2025 + (Sequencing Cost) | $4050 + (Sequencing Cost) |
| GEM-X Universal Gene Expression On-Chip-Multiplex (4-plex) | $887 + (Sequencing Cost) | $1774 + (Sequencing Cost) |
| GEM-X Flex Gene Expression singleplex | $2078 + (Sequencing Cost) | $4156 + (Sequencing Cost) |
| GEM-X Flex Gene Expression multiplex | $1103 + (Sequencing Cost) | $2206 + (Sequencing Cost) |
| GEM-X VDJ Enrichment | $111 + (10X Capture) + (Sequencing Cost) | $222 + (10X Capture) + (Sequencing Cost) |
| GEM-X Feature Barcode | $130 + (10X Capture) + (Sequencing Cost) | $260 + (10X Capture) + (Sequencing Cost) |
| Single Cell Illumina PIPSeq Library Construction | NCI Price | Non NCI Price |
|---|---|---|
| Project Type | Cost/Sample | Cost/Sample |
| Illumina PIPseq T2 | $324 + (Sequencing Cost) | $648 + (Sequencing Cost) |
| Illumina PIPseq T10 | $692 + (Sequencing Cost) | $1384 + (Sequencing Cost) |
| Illumina PIPseq T20 | $954 + (Sequencing Cost) | $1908 + (Sequencing Cost) |
| Illumina PIPseq T100 | $3369 + (Sequencing Cost) | $6738 + (Sequencing Cost) |
| Single Cell QC Run | |
|---|---|
| Number of libraries | QC Run Type Required |
| 1 | N/A |
| 2-6 | NextSeq P1 |
| 7-12 | NextSeq P2 |
| 13+ | NovaSeq 1.5B |
| Long Read Sequencing Services Pacbio Library Construction | NCI Price | Non NCI Price |
|---|---|---|
| Project Type | Cost/Sample | Cost/Sample |
| Amplicon | $107 + (Sequencing Cost) | $214 + (Sequencing Cost) |
| Bulk Iso-seq V2 | $174 + (Sequencing Cost) | $348 + (Sequencing Cost) |
| Bulk Kinnex Iso-seq | $522 + (Sequencing Cost) | $1044 + (Sequencing Cost) |
| WGS | $151 + (Sequencing Cost) | $302 + (Sequencing Cost) |
| Single Cell Kinnex Iso-seq* | $448 + (Sequencing Cost) | $896 + (Sequencing Cost) |
| * Single cell capture is not included | ||
| Pacbio Sequencing | NCI Price | Non NCI Price |
|---|---|---|
| Project Type | Cost/SMRT Cell | Cost/Flowcell |
| Revio SPRQ SMRT Cell | $1,087 | $2,174 |
| Oxford Nanopore Library Construction | NCI Price | Non NCI Price |
|---|---|---|
| Project Type | Cost/Sample | Cost/Sample |
| WGS and Adaptive sampling | $188 + (Sequencing Cost) | $376 + (Sequencing Cost) |
| Direct RNA-Seq | $174 + (Sequencing Cost) | $348 + (Sequencing Cost) |
| Ultra-Long | $212 + (Sequencing Cost) | $424 + (Sequencing Cost) |
| RNA-seq (cDNA-PCR) | $203 + (Sequencing Cost) | $406 + (Sequencing Cost) |
| Single cell transcriptomics | $209 + (Sequencing Cost) | $418 + (Sequencing Cost) |
| Oxford Nanopore Sequencing | NCI Price | Non NCI Price |
|---|---|---|
| Project Type | Cost/Flowcell | Cost/Flowcell |
| Nanopore flow cell | $1,092 | $2,184 |
| 1 Wash and Reload* | $32 | $64 |
| *Wash and Reload is for WGS samples, will wash and reload up to 2 times | ||
Sequencing Cost Calculator
*For full estimate, including library prep., etc., click "Next".
| Sample_ID | Library Type | Target Cell Number (in thousands) | Target Reads per Cell (in thousands) | Number of Reads per Sample (in millions) | Total Reads (in millions) |
|---|
*For full estimate, including library prep., etc., click "Next".
*For full estimate, including library prep., washing kits, etc., click "Next".
*For full estimate, including library prep., washing kits, etc., click "Next".
| Sample_ID | Library Type | Target Cell Number (in thousands) | Target Reads per Cell (in thousands) | Number of Reads per Sample (in millions) | Total Reads (in millions) |
|---|
*For full estimate, including library prep., qc, etc., click "Next".
| Sample_ID | Library Type | Target Cell Number (in thousands) | Target Reads per Cell (in thousands) | Number of Reads per Sample (in millions) | Total Reads (in millions) |
|---|
*For full estimate, including library prep., qc, etc., click "Next".
Add-On Modalities (Optional)
Add-On Modalities (Optional)
Add-On Modalities (Optional)
| Description | Unit | Quantity | Total | |
|---|---|---|---|---|
| Library Prep | ||||
| Kit | ||||
| QC CostPooling Cost | QCPooling | 1 | ||
| Grand Total: | ||||
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* You have an option to request a QC run. But we do not guarantee the read distribution if you send us already pooled libraries. |
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* This is only an estimate, generated on October 17, 2026 at 2:50 pm. Please reach out to Bao Tran (tranb2@mail.nih.gov) or Jyoti Shetty (jyoti.shetty@nih.gov) for a more accurate quote. |
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| Description | Unit | Quantity | Total | |
|---|---|---|---|---|
| Library Prep | ||||
| Nanopore flow cell | Consumable | |||
| Wash and Reload | Consumable | |||
| Grand Total: | ||||
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* This is only an estimate, generated on October 17, 2026 at 2:50 pm. Please reach out to Bao Tran (tranb2@mail.nih.gov) or Kayla Kline (kayla.eury@nih.gov) for a more accurate quote. |
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| Description | Unit | Quantity | Total | |
|---|---|---|---|---|
| Library Prep | ||||
| Revio SMRT Cell | Consumable | |||
| Grand Total: | ||||
|
* This is only an estimate, generated on October 17, 2026 at 2:50 pm. Please reach out to Bao Tran (tranb2@mail.nih.gov) or Kayla Kline (kayla.eury@nih.gov) for a more accurate quote. |
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| Item | Type | Quantity | Unit Price | Total |
|---|---|---|---|---|
| Grand Total: | ||||
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* This is only an estimate, generated on October 17, 2026 at 2:50 pm. Please reach out to Bao Tran (tranb2@mail.nih.gov) or Lirong Peng (Lirong.peng@nih.gov) for a more accurate quote. |
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