Here you will find all the forms necessary for submitting your sequencing proposal and samples to the laboratory. To aid in project planning, we have also provided handouts of the technical details of each sequencing platform as well as the sample preparation protocols used by our laboratory. Do you have additional questions about the Next-Gen Sequencing Group? Check out our sequencing FAQs, containing the most common questions we receive!
Laboratory Forms and Information
Single Cell
PacBio
Protocols and Resources
Illumina
- Sample processing workflow
- Illumina sequencing primer
- Please contact Illumina Technical Support techsupport@illumina.com for up-to-date information
- Illumina’s Sample Preparation Protocols:
Single Cell
10X Genomics
PacBio
- Sample processing workflow
- PacBio Sequencing Protocols:
- DNA Extraction Recommendations:
- SMRT Sequencing Information:
ONT
- ONT Sequencing Protocols:
- ONT Instruments:
Sequencing Cost Calculator
*For full estimate, including library prep., etc., click "Next".
*For full estimate, including library prep., washing kits, etc., click "Next".
*For full estimate, including library prep., washing kits, etc., click "Next".
* QC run is required for 12 or more samples.
*For full estimate, including library prep., etc., click "Next".
* QC run is required for 12 or more samples.
*For full estimate, including library prep., etc., click "Next".
Add-On Modalities (Optional)
Add-On Modalities (Optional)
| Description | Unit | Quantity | Total | |
|---|---|---|---|---|
| Library Prep | ||||
| Kit | ||||
| QC CostPooling Cost | QCPooling | 1 | $1,228.00 | $1,228.00 |
| Grand Total: | ||||
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* You have an option to request a QC run. But we do not guarantee the read distribution if you send us already pooled libraries. |
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* This is only an estimate, generated on September 9, 2025 at 12:30 am. Please reach out to Bao Tran (tranb2@mail.nih.gov) or Jyoti Shetty (jyoti.shetty@nih.gov) for a more accurate quote. |
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| Description | Unit | Quantity | Total | |
|---|---|---|---|---|
| Library Prep | ||||
| Nanopore flow cell | Consumable | |||
| Washing kit | Consumable | |||
| Reloading reagents | Consumable | |||
| Grand Total: | ||||
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* This is only an estimate, generated on September 9, 2025 at 12:30 am. Please reach out to Bao Tran (tranb2@mail.nih.gov) or Juan Manuel Caravaca-Guasch (juanmanuel.carava@nih.gov) for a more accurate quote. |
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| Description | Unit | Quantity | Total | |
|---|---|---|---|---|
| Library Prep | ||||
| Revio SMRT Cell | Consumable | |||
| Grand Total: | ||||
|
* This is only an estimate, generated on September 9, 2025 at 12:30 am. Please reach out to Bao Tran (tranb2@mail.nih.gov) or Juan Manuel Caravaca-Guasch (juanmanuel.carava@nih.gov) for a more accurate quote. |
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| Item | Type | Quantity | Unit Price | Total |
|---|---|---|---|---|
| Grand Total: | ||||
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* This is only an estimate, generated on September 9, 2025 at 12:30 am. Please reach out to Bao Tran (tranb2@mail.nih.gov) or Yunlong He (yunlong.he2@nih.gov) for a more accurate quote. |
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